Ultra-high-specificity enzyme substrates:
Artificial enzyme substrates (protease and kinase substrate peptides, etc.)
have been the key to many advances in modern biology and biotechnology.
Yet, useful as they are, many of these substrates have sub-optimal
specificity. There is too much cross-reaction with non-target enzymes.
This makes the analysis of complex biochemical pathways difficult. It
makes the creation of proteomic microarrays for complex enzymatic pathways
Molecular Pathways has devised
generalized methods for rapidly developing new, ultra-high-specificity,
enzyme substrates patent
7,291,698, (a CIP, 11/872,012
which covers both enzyme substrates and enzyme inhibitors,
is currently pending). These ultra-high-specificity substrates may be
used for improved proteomic microarrays, traditional microwell assays, or
any application where improved specificity is desired.
Diagram showing a
matrix metalloprotein artifical peptide substrate (RPFS-MIMG) and Molecular
Pathway's proprietary "steric blocker" groups (blue chains).
blocker" groups extend beyond the MMP-3 enzyme's active site. They
prevent some MMP's from getting access to the substrate, and thus increase
These same concepts can also be used to
produce higher specificity enzyme inhibitors, which could potentially be useful
for higher specificity drugs and drug candidates. Such inhibitors might
exhibit fewer unwanted side effects. This application is pending.
presentation with animated graphics, illustrating the concept, can be