Molecular Pathway's technology (patent
(text) also enables simple, high performance, immunochemical test strips and
arrays that produce easy to detect electrochemical signals. This produces
extremely simple assays that can be read by simple, low-cost meters, suitable
for field use and home medical instrumentation. A CIP of this patent (20070289880)
focusing on enzymatic and coagulation assays is in progress.
reactivation immunochemical sensor is similar to a conventional blood glucose
test-strip, with the exception that the strip uses apoglucose oxidase instead of
glucose oxidase. The apoglucose oxidase is enzymatically inactive because
its cofactor, FAD has been removed. Here the FAD cofactor is conjugated
with an antigen, and this antigen is, in-turn, bound to an antibody.
When excess test
antigens are added, the test-antigens displace the bound FAD from the antibody.
This FAD reactivates apoglucose oxidase. The reactivated glucose oxidase
then reacts with glucose (present in the test chemistry), producing an
electrochemical signal that is detected by the test strip's electrodes.
In contrast to other proposed
electrochemical immunosensor technologies (reviewed
here), the apoenzyme reactivation technology is relatively simple,
and leverages off of the large amount of electrochemical blood glucose
test-strip infrastructure. An additional advantage is that since
we have an IP licensing focus, our apoenzyme reactivation
electrochemical technology is both available and affordable.